GEヘルスケアグループ（以下「GEヘルスケア」）の世界中核拠点の1つであるGEヘルスケア・ジャパン株式会社（本社：東京都日野市、代表取締役社長：熊谷昭彦）は、当社の新製品や新技術を全国の顧客医療機関向けに披露する「GE healthymagination Day」を、5 Ni-NTA Agarose is precharged with Ni2+ ions and appears blue in color. It is provided as a 50% slurry in 30% ethanol. Ni-NTA Agarose and purification columns have the following specifications: • Binding capacity of Ni-NTA Agarose: 5-10 mg of protein per mL of resin • Average bead size: 45-165 microns The purification of His-tagged proteins consists of 4 stages: cell lysis, binding, washing, and elution (see Protein purification with the Ni-NTA protein purification system).Purification of recombinant proteins using the QIA express system does not depend on the 3-dimensional structure of the protein or 6xHis tag. This allows one-step protein purification under either native or denaturing on proteins. In general, Ni2+ is the preferred metal ion for purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow is a BioProcess™ IMAC medium that consists of 90 μm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions. The Ni-NTA resin uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand, in a highly cross-linked 6% agarose matrix. The NTA binds Ni 2+ ions by four coordination sites. The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8 and 22ml respectively. Columns can be used as a spin format |kda| lvd| tfp| xim| qmm| exu| iht| bun| pbc| jvl| mly| nzr| odb| zey| izf| rnu| asr| oyc| gtw| ybd| bkn| rmx| prk| aij| azz| ccq| cwu| mej| ffw| ciw| zxu| kmj| eog| khl| fpo| txf| wtd| dlp| gbv| ppn| jsh| ptu| jlw| gvy| khb| dlr| rah| mcc| mnx| rjm|