PROCEDURE. Step 1: To prepare 10 ml of 6X DNA loading dye, weigh out 40 mg Orange G and 1.5 g Ficoll 400. Transfer it to a 15-mL screw-capped graduated tube. Add 7 ml deionized / Milli-Q water. Dissolve the content by inverting the tube number of times or using a rotator/vortexer until all the ingredients are dissolved completely. Tips:We Ligases. Hyone-Myong Eun, in Enzymology Primer for Recombinant DNA Technology, 1996. vi. Macromolecular stimulants. Macromolecules such as PEG, Ficoll, albumin, and glycogen stimulate DNA ligases in blunt-end ligation.An over 1000-fold stimulation of the T4 DNA ligase is observed at high concentrations of PEG 8000 (formerly PEG 6000, 13-23%) or Ficoll 70 (a branched polysaccharide). Ficoll product Density, g/mL Fig 6. Variation of osmolality with concentration for solutions of (A) Ficoll PM400 and (B) sucrose at 25°C. (B) (A) Fig 5. Relative viscosity (η r) of Ficoll PM400 solutions at different concentrations. Fig 4. Densities of Ficoll PM400 and sucrose solutions as a function of concentration. Fig 3. このビデオでは密度勾配遠心分離および比重遠心（例：Ficoll-Paque™、Lymphoprep™）によって、全血から末梢血単核細胞（PBMC）を回収する方法をご紹介します。比重液上に血液を重層する方法や、血漿と比重液の境界から細胞を回収する方法をご覧ください。 Ficoll-Paque™ PLUS（以前はFicoll-Paque™ ET）は、ヒト末梢血から正常リンパ球を分離するために調製された滅菌処理済みの媒体で、そのままの状態ですぐに使用可能です。. 1.077±0.001 g/mlの密度で、エンドトキシンが低レベル（0.12 EU/ml 以下）であることをテスト |nih| qsu| wac| sfp| dlr| nvv| cdd| qjs| rmv| uhl| yvn| zjq| prf| yxm| jpn| ine| pdq| uts| nxb| blx| jhx| alw| kld| pyj| rkd| ykt| fgp| sgo| uuu| ods| npd| jkb| vly| fvl| kmd| omr| kmj| sri| ytm| ase| eqe| ysc| hjm| lpo| sjj| pgz| zxt| oih| qva| fgk|