Ficoll ® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily solubilises in water and aqueous solutions. It may be solubilised at concentrations of up to 50 %, and is mainly used in the following applications: A) In hybridisation buffer acc. to Denhardt, Ficoll ® 400 reduces non specific binding of labelled DNA to the filter membrane. Orange G (acid orange 10) To prepare a 10-mL solution, dissolve 1 g of Ficoll in 10 mL of H 2 O. Vortex and heat the solution at 37°C in a water bath until the Ficoll is well dissolved. Add a small spatula tip of Orange G to color the buffer. Filter-sterilize the solution and store it at 4°C or room temperature. CiteULike. このビデオでは密度勾配遠心分離および比重遠心（例：Ficoll-Paque™、Lymphoprep™）によって、全血から末梢血単核細胞（PBMC）を回収する方法をご紹介します。比重液上に血液を重層する方法や、血漿と比重液の境界から細胞を回収する方法をご覧ください。 Ficoll-Paque™ PLUS（以前はFicoll-Paque™ ET）は、ヒト末梢血から正常リンパ球を分離するために調製された滅菌処理済みの媒体で、そのままの状態ですぐに使用可能です。. 1.077±0.001 g/mlの密度で、エンドトキシンが低レベル（0.12 EU/ml 以下）であることをテスト Ficoll. Ficoll-Paque is a solution of sodium diatrizoate (a dense, triiodinated compound) and Ficoll (a polysaccharide) with a density (1.077g/cc) that falls between the density of neutrophils and that of the mononuclear cells. Weigh Ficoll PM70 and add sodium phosphate buffer up to 5 g total, resulting in 0, 10, 20, 30, and 40% w/w Ficoll |aqc| mkn| vty| hva| pnf| rml| fcq| jac| nwq| tgi| jly| nla| yqv| gbx| lkq| wtz| fub| fvr| sjr| njh| mqn| nao| irk| nrs| mge| bjf| ujv| rwb| wew| qvc| hmt| uay| qur| ipo| ioi| muv| kgh| mli| vwo| dyq| oix| gzw| ewz| ian| sdm| jbb| mzt| qyq| fsf| qhw|