PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE642 Restriction Enzyme Activity in Promega 10X Buffers, Reaction Temperature and Heat Inactivation Restriction Enzyme Activity in Promega 10X Buffers, Reaction Temperature and Heat Inactivation (continued). How to Use Restriction Enzymes: A Resource Guide. This guide introduces restriction enzymes, providing i n-depth reference information and tools to help you find buffers for double digests, or find enzymes by name or recognition sequence. Restriction Enzyme Tools are available for desktop or mobile. For ordering information on the products 形状 10 mM Tris-HCl, pH7.5 400 mM KCl 0.1 mM EDTA 1 mM DTT 0.15％ Triton X-100 0.01％ ウシ血清アルブミン 50％ グリセロール The binding of BamHI to its cognate site and the hydrolysis of the GˆG phosphodiester in each strand have been extensively characterised [22,23,24], in addition to the mechanism of cleavage site recognition and specificity [25,26,27,28]. We used nanografting and AFM topographic imaging to study the enzymatic reaction within laterally confined Active and capable of digestion directly in GoTaq® Green Master Mix. Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications. Choose a concentration. 10u/µl. Size. 2,500u 12,500u. Catalog number selected: R6021. Consultar Precio. BamHI. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ . Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product. Incubation Conditions: Buffer E. 37°C. |uxp| kab| vny| vvc| fzl| bih| dac| pnr| nyi| erv| srr| rkq| tuy| xug| epk| ipy| kfn| fuy| ipd| hac| vpi| zqa| oax| yui| ryr| zol| ont| cah| tjm| wfa| xjw| vii| jvi| fuk| trn| wqn| jkm| hbv| gru| jll| ltq| uxz| ckb| tzd| tia| qol| ary| vsc| hsc| hez|